In many resource limited countries, availability of cost effective instruments is a major problem. Hence accurate analysis cannot be done. Due to its high cost, HPLC is lacking in such countries. Again the cost of the column, solvents and time required for multiple analysis as well as mobile phase optimization is more. Therefore HPTLC is a good option over HPLC in terms of time as well as overall cost. Metformin HCl and glibenclamide are available in combined dosage form in market. Present study attempted for development and validation of high performance thin layer chromatography (HPTLC) method for simultaneous estimation of metformin HCl and glibenclamide using densitometric detection. Separation was performed using camag HPTLC system on silica gel 60F254 plates. The mobile phase was comprising of toluene: ethanol: formic acid (4.5:4.5:1.0 v/v/v). The detection wavelength was 230 nm. The Rf values of metformin HCl and glibenclamide were found to be 0.24±0.03 and 0.82±0.04, respectively. The method was validated as per ICH guidelines. A F-test indicated that calibration graphs were adequately linear at the evaluated concentration ranges. The pooled % RSD for repeatability of the percentage amount recovered for metformin HCl and glibenclamide were found to be 0.670 and 1.022. The percentage recoveries for the trueness were 99.86%±1.5 for metformin HCl and 99.61%±1.7 for glibenclamide (n = 3). The developed HPTLC method was found to be new, accurate, sensitive, precise and less time consuming as compare to published method. Thus it was successfully used to analyze fixed-dose tablets samples of metformin HCl and glibenclamide.
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